Search results for " low pH"
Article
Industry Adoption of Single-Use Systems Remains Low
Single-use technologies are starting to gain ground as capacity needs change, but industrywide adoption remains low.
By Feliza Mirasol
zli…
Article
Efficient concentration of a low-titer bovine IgG with high recovery in low volume
This application note describes the design of circuits with low working volume, allowing for high concentration of dilute feed streams. The use of ReadyCircuit™ single-use assemblies allowed for as…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
A purification process was developed for an acidic Fc-fusion protein that was unstable at ambient temperatures and low pH and that quickly formed high molecular weight oligomers (HMW1) and dimers (HMW…
Article
Determining Criticality-Process Parameters and Quality Attributes Part I: Criticality as a Continuum
A practical roadmap in three parts that applies scientific knowledge, risk analysis, experimental data, and process monitoring throughout the three phases of the process validation lifecycle.
A…
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Development of Purification for Challenging Fc-Fusion Proteins
A low pH buffer is routinely used to elute the mAb product, which is followed by a viral inactivation step.
Anion exchange chromatography (AEX) (6-8, 10-11), typically serves as a polishi…
Article
Host-Cell Protein Measurement and Control
Jun 01, 2015
By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem
BioPharm International
Volume 28, Issue 6, pg 32–38
Host-cell proteins (HCPs) constitute a major part of proce…
Article
Evolution of the Monoclonal Antibody Purification Platform
The low pH elution is another potential issue as it has been linked to product aggregation.
• Low pH viral inactivation as an orthogonal step for clearance of retroviruses. This step also suff…
Article
Selecting the Right Viral Clearance Technology
Ideally, methods from the first category, which include inactivation mechanisms such as low pH, detergent, or temperature hold steps and robust removal steps, such as virus filtration that clear a bro…
Article
Optimization of Protein Expression in Escherichia Coli
May 01, 2015
By Siavash Bashiri, David Vikström, Nurzian Ismail
BioPharm International
Volume 28, Issue 5
Production of proteins for manufacturing therapeutics and pharmaceuticals is a c…
Article
A Closer Look at Affinity Ligands
…bzena): One of the challenges of traditional protein A affinity chromatography is the use of acidic, low pH, elution. The low pH can lead to instability of certain antibodies potentially causing aggr…